Effect of water-miscible organic solvents on the catalytic activity of penicillin acylase from Kluyvera citrophilaGUISAN, J. M; ALVARO, G; BLANCO, R. M et al.Studies in organic chemistry. 1987, Vol 29, pp 331-336, issn 0165-3253Conference Paper

The coimmobilization of D-amino acid oxidase and catalase enables the quantitative transformation of D-amino acids (D-phenylalanine) into α-keto acids (phenylpyruvic acid)FERNANDEZ-LAFUENTE, R; RODRIGUEZ, V; GUISAN, J. M et al.Enzyme and microbial technology. 1998, Vol 23, Num 1-2, pp 28-33, issn 0141-0229Article

Stabilization of micrococcal endonuclease by immobilization on agarose gels highly activated with CNBrGUISAN, J. M; SERRANO, J; BALLESTEROS, A et al.Biocatalysis. 1993, Vol 8, Num 1, pp 81-89, issn 0886-4454Article

Stabilisation of enzymes by multipoint covalent attachment to agarose-aldehyde gels. Borohydride reduction of trypsin-agarose derivativesBLANCO, R. M; GUISAN, J. M.Enzyme and microbial technology. 1989, Vol 11, Num 6, pp 360-366, issn 0141-0229Article

Organic reactions catalyzed by insolubilized enzymes : I-peptide synthesis catalyzed by insolubilized α-chymotrypsinSALVADOR, D; SINISTERRA, J. V; GUISAN, J. M et al.Journal of molecular catalysis. 1990, Vol 62, Num 1, pp 93-105, issn 0304-5102Article

Polyethyleneimine (PEI) functionalized ceramic monoliths as enzyme carriers : Preparation and performanceDE LATHOUDER, K. M; VAN BENTHEM, D. T. J; WALLIN, S. A et al.Journal of molecular catalysis. B, Enzymatic. 2008, Vol 50, Num 1, pp 20-27, issn 1381-1177, 8 p.Article

Detection of polyclonal antibody against any area of the protein-antigen using immobilized protein-antigens : The critical role of the immobilization protocolFUENTES, Manuel; MATEO, Cesar; FERNANDEZ-LAFUENTE, Roberto et al.Biomacromolecules. 2006, Vol 7, Num 2, pp 540-544, issn 1525-7797, 5 p.Article

Preparation of new lipases derivatives with high activity-stability in anhydrous media : adsorption on hydrophobic supports plus hydrophilization with polyethylenimineGUISAN, J. M; SABUQUILLO, P; FERNANDEZ-LAFUENTE, R et al.Journal of molecular catalysis. B, Enzymatic. 2001, Vol 11, Num 4-6, pp 817-824, issn 1381-1177Conference Paper

Affinity chromatography of plasma proteins (guanidinobenzoatase) : use of mimetic matrices and mimetic soluble ligands to prevent the binding of albumin on target affinity matricesMURZA, A; AGUILAR, A. R; FERNANDEZ-LAFUENTE, R et al.Journal of chromatography. B, Biomedical sciences and applications. 1999, Vol 732, Num 1, pp 165-172, issn 1387-2273Article

Modulation of the properties of penicillin G acylase by acyl donor substrates during N-protection of amino compoundsFERNANDEZ-LAFUENTE, R; ROSELL, C. M; GUISAN, J. M et al.Enzyme and microbial technology. 1998, Vol 22, Num 7, pp 583-587, issn 0141-0229Article

Resolution of racemic mixtures by synthesis reactions catalyzed by immobilized derivatives of the enzyme penicillin G acylaseROSELL, C. M; FERNANDEZ-LAFUENTE, R; GUISAN, J. M et al.Journal of molecular catalysis. 1993, Vol 84, Num 3, pp 365-371, issn 0304-5102Conference Paper

Organic reactions catalyzed by insolubilized enzymes. II, Influence of structure of the acyl donor in selectivity of α-chymotrypsinCABEZAS, M. J; DEL CAMPO, C; LLAMA, E et al.Journal of molecular catalysis. 1990, Vol 62, Num 3, pp 353-367, issn 0304-5102Article

Reversible and strong immobilization of proteins by ionic exchange on supports coated with sulfate-dextranFUENTES, Manuel; PESSELA, Benevides C. C; GUISAN, J. M et al.Biotechnology progress. 2004, Vol 20, Num 4, pp 1134-1139, issn 8756-7938, 6 p.Article

Design of new immobilized-stabilized carboxypeptidase A derivative for production of aromatic free hydrolysates of proteinsTARDIOLI, P. W; FERNANDEZ-LAFUENTE, R; GUISAN, J. M et al.Biotechnology progress. 2003, Vol 19, Num 2, pp 565-574, issn 8756-7938, 10 p.Article

Regioselective hydrolysis of peracetylated α-D-glucopyranose catalyzed by immobilized lipases in aqueous medium. A facile preparation of useful intermediates for oligosaccharide synthesisBASTIDA, A; FERNANDEZ-LAFUENTE, R; FERNANDEZ-LORENTE, G et al.Bioorganic & medicinal chemistry letters (Print). 1999, Vol 9, Num 4, pp 633-636, issn 0960-894XArticle

Stabilization of enzymes (D-amino acid oxidase) against hydrogen peroxide via immobilization and post-immobilization techniquesFERNANDEZ-LAFUENTE, R; RODRIGUEZ, V; MATEO, C et al.Journal of molecular catalysis. B, Enzymatic. 1999, Vol 7, Num 1-4, pp 173-179, issn 1381-1177Conference Paper

Stabilization of multimeric enzymes via immobilization and post-immobilization techniquesFERNANDEZ-LAFUENTE, R; RODRIGUEZ, V; MATEO, C et al.Journal of molecular catalysis. B, Enzymatic. 1999, Vol 7, Num 1-4, pp 181-189, issn 1381-1177Conference Paper

Immobilization-stabilization of thermolysin onto activated agarose gelsGUISAN, J. M; POLO, E; AGUADO, J et al.Biocatalysis and biotransformation (Print). 1997, Vol 15, Num 3, pp 159-173, issn 1024-2422Article

Stabilization of heterodimeric enzyme by multipoint covalent immobilization : penicillin G acylase from Kluyvera citrophilaGUISAN, J. M; ALVARO, G; FERNANDEZ-LAFUENTE, R et al.Biotechnology and bioengineering. 1993, Vol 42, Num 4, pp 455-464, issn 0006-3592Article

The equilibrium and kinetics of N-acetyl-tryptophan phenylethyl ester synthesis by agarose-chymotrypsin in organic mediaBLANCO, R. M; GUISAN, J. M; HALLING, P. J et al.Biotechnology and bioengineering. 1992, Vol 40, Num 9, pp 1092-1096, issn 0006-3592Article

Enzyme reaction engineering : synthesis of antibiotics catalyzed by stabilized penicillin G acylase in the presence of organic cosolventsFERNANDEZ-LAFUENTE, R; ROSELL, C. M; GUISAN, J. M et al.Enzyme and microbial technology. 1991, Vol 13, Num 11, pp 898-905, issn 0141-0229Article

Enzymatic β-galactosidation of β-xylopyranosidesLOPEZ, R; FERNANDEZ-MAYORALAS, A; MARTIN-LOMAS, M et al.Biotechnology letters. 1991, Vol 13, Num 10, pp 705-710, issn 0141-5492Article

Immobilization-stabilization of penicillin G acylase from Escherichia coliALVARO, G; FERNANDEZD-LAFUENTE, R; BLANCO, R. M et al.Applied biochemistry and biotechnology. 1990, Vol 26, Num 2, pp 181-195, issn 0273-2289, 15 p.Article

Purification, immobilization, and stabilization of a lipase from Bacillus thermocatenulatus by interfacial adsorption on hydrophobic supportsPALOMO, J. M; SEGURA, R. L; FERNANDEZ-LORENTE, G et al.Biotechnology progress. 2004, Vol 20, Num 2, pp 630-635, issn 8756-7938, 6 p.Article

The role of 6-aminopenicillanic acid on the kinetics of amoxicillin enzymatic synthesis catalyzed by penicillin G acylase immobilized onto glyoxyl-agaroseGONCALVES, L. R. B; FERNANDEZ-LAFUENTE, R; GUISAN, J. M et al.Enzyme and microbial technology. 2002, Vol 31, Num 4, pp 464-471, issn 0141-0229, 8 p.Article